Best Embryo Freezing Cryopreservation in Kolkata

What is Embryo Freezing Cryopreservation and How Does It Work?

Sparsha IVF offers a cutting-edge fertility preservation program known as Oocyte Cryopreservation, commonly referred to as egg freezing. This advanced IVF technique allows women with a healthy ovarian reserve to freeze their high-quality eggs for future use.
Sparsha IVF understands the importance of preserving fertility, whether for medical reasons such as impending treatments that may affect ovarian function or for personal decisions like delaying childbearing for career or other social reasons. Dr. Debalina Brahma, a leading Fertility Specialist at Sparsha IVF, is renowned for providing top-notch cryopreservation services.
It is recommended to consider egg freezing at a younger age, ideally before 34, to ensure the best quality eggs are preserved for future fertility treatments.

Applications of Cryopreservation in Embryo Preservation

In the context of infertility treatment, cryopreservation is commonly used to store embryos that are not immediately transferred during in vitro fertilization (IVF) procedures. Embryo cryopreservation allows patients to have the option of using these embryos in future IVF cycles, offering increased flexibility and higher chances of successful conception.

Overview of the Cryopreservation Process

The cryopreservation process begins with the careful preparation of the cells or tissues to be frozen. The cells are then slowly cooled to a low temperature, and cryoprotective agents are often used to protect them from damage during freezing.

Once the cells are sufficiently cooled using a strict cryopreservation protocol, they are transferred to storage containers filled with liquid nitrogen for freezing and thawing, where they can be preserved for extended periods.

Principle of Cryopreservation Method

• To generalise the process cryopreservation is a method of preserving cells, organs, or any other biological matter to save them from damage and to keep them ready for future use. Here the material is stored at extremely low temperatures, around -80°C with carbon dioxide or -196°C with the use of liquid nitrogen.
• Here, extremely low temperatures are used for cryopreserving living tissues, like sperm and stem cells, to keep them safe for future use; unprotected freezing in this manner is typically very lethal.
• The entire process of cryopreservation is based on the chemical conversion of water present in cells (including the fluid in cell membranes) to a solidified state, often through freezing and thawing.
• When we apply a cryopreservation protocol by cooling something below 0 degrees, freezing water (which typically constitutes 80% of tissue mass) dominates the biological effects.
• Cell liquid or water in the cell membrane requires lower temperatures, as seen in cryopreservation, as compared to normal water due to the presence of salts and other chemical and organic molecules.
• Such low temperatures almost stop any chances of any metabolic activity or biological divisions taking place.

Process of Cryopreservation

Following are the steps which are followed for the regeneration & culturing of plant cells. Initially, aseptic tissue cultures are produced. Subsequently, cryoprotectants along with pre-processing chemicals are incorporated. The process then continues with the succeeding steps being freezing, storing, thawing, and re-culturing in the aforementioned order. Moreover, the practicality of the derived product is consequently evaluated. Upon the completion of these phases, the plant then goes through a renewal process. Embryos are preserved in cryogenic containers at Sparsha IVF, which are consistently checked.

Benefits and Applications of Embryo Cryopreservation